pmir-report firefly luciferase vector Search Results


pmirreport firefly luciferase (ffl) vector  (Promega)
90
Promega pmirreport firefly luciferase (ffl) vector
Pmirreport Firefly Luciferase (Ffl) Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmir-report+firefly+luciferase+vector/pmirreport+firefly+luciferase++ffl++vector/pm22265971-32-34-39
Average 90 stars, based on 1 article reviews
pmirreport firefly luciferase (ffl) vector - by Bioz Stars, 2026-10
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firefly luciferase expression vectors pmir-report  (Promega)
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Promega firefly luciferase expression vectors pmir-report
Firefly Luciferase Expression Vectors Pmir Report, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmir-report+firefly+luciferase+vector/firefly+luciferase+expression+vectors+pmir+report/pmc07272390-82-13-18
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firefly luciferase expression vectors pmir-report - by Bioz Stars, 2026-10
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firefly luciferase reporter vector pmir-repotr  (Promega)
90
Promega firefly luciferase reporter vector pmir-repotr
Firefly Luciferase Reporter Vector Pmir Repotr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmir-report+firefly+luciferase+vector/firefly+luciferase+reporter+vector+pmir+repotr/pmc04281840-75-33-38
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firefly luciferase reporter vector pmir-repotr - by Bioz Stars, 2026-10
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pmir-report firefly luciferase transfection control vector  (Promega)
90
Promega pmir-report firefly luciferase transfection control vector
<t>Transfection</t> of miR-518f-5p mimic increases breast cell migration and decreases CD9 protein levels. ( A ). Cell migration in 184A1 cells following transfection of a miR-518f-5p mimic assessed using transwell migration assay. ( B ). Migration of MDA-MB-231 cells following transfection of miR-518f-5p mimic. Migration assay results are shown as fluorescence intensity (arbitrary units), * p = 0.02, ** p = 0.002. ( C ). Proliferation of 184A1 cells using the resazurin assay, after transfection of miR-518f-5p mimic. ( D ). MDA-MB-231 proliferation following transfection of a miR-518f-5p mimic, as measured using resazurin assay. Cell proliferation results are shown as fold change fluorescence relative to 24 h fluorescence (arbitrary units). ( E ). Changes in cell adhesion to basement membrane extract (BME) and fibronectin (FN) were assessed using an adhesion assay, following transfection with miR-518f-5p mimic in 184A1 cells. ( F ). Cell adhesion was assessed in MDA-MB-231 cells following transfection of miR-518f-5p mimic. Adhesion results are expressed as a ratio of adhesion (fluorescence intensity of adherent cells compared to total seeded cells, arbitrary units), FN: * p = 0.04, BME: * p = 0.01, n = 3. ( G ). Total CD9 protein expression was measured using SDS-PAGE and western blotting, following transfection of a miR-518f-5p mimic in 184A1 (left) and MDA-MB-231 cells (right) for 72 h. ( H ). Representative western blots of CD9 total protein expression following transfection with miR-518f-5p mimic for 72 h. Results shown as CD9 total protein normalized to beta actin protein expression, n = 3, * p = 0.04, ** p = 0.001. See for full western blotting images.
Pmir Report Firefly Luciferase Transfection Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmir-report+firefly+luciferase+vector/pmir+report+firefly+luciferase+transfection+control+vector/pmc07226392-143-26-32
Average 90 stars, based on 1 article reviews
pmir-report firefly luciferase transfection control vector - by Bioz Stars, 2026-10
90/100 stars
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Transfection of miR-518f-5p mimic increases breast cell migration and decreases CD9 protein levels. ( A ). Cell migration in 184A1 cells following transfection of a miR-518f-5p mimic assessed using transwell migration assay. ( B ). Migration of MDA-MB-231 cells following transfection of miR-518f-5p mimic. Migration assay results are shown as fluorescence intensity (arbitrary units), * p = 0.02, ** p = 0.002. ( C ). Proliferation of 184A1 cells using the resazurin assay, after transfection of miR-518f-5p mimic. ( D ). MDA-MB-231 proliferation following transfection of a miR-518f-5p mimic, as measured using resazurin assay. Cell proliferation results are shown as fold change fluorescence relative to 24 h fluorescence (arbitrary units). ( E ). Changes in cell adhesion to basement membrane extract (BME) and fibronectin (FN) were assessed using an adhesion assay, following transfection with miR-518f-5p mimic in 184A1 cells. ( F ). Cell adhesion was assessed in MDA-MB-231 cells following transfection of miR-518f-5p mimic. Adhesion results are expressed as a ratio of adhesion (fluorescence intensity of adherent cells compared to total seeded cells, arbitrary units), FN: * p = 0.04, BME: * p = 0.01, n = 3. ( G ). Total CD9 protein expression was measured using SDS-PAGE and western blotting, following transfection of a miR-518f-5p mimic in 184A1 (left) and MDA-MB-231 cells (right) for 72 h. ( H ). Representative western blots of CD9 total protein expression following transfection with miR-518f-5p mimic for 72 h. Results shown as CD9 total protein normalized to beta actin protein expression, n = 3, * p = 0.04, ** p = 0.001. See for full western blotting images.

Journal: Cancers

Article Title: Tetraspanin CD9 is Regulated by miR-518f-5p and Functions in Breast Cell Migration and In Vivo Tumor Growth

doi: 10.3390/cancers12040795

Figure Lengend Snippet: Transfection of miR-518f-5p mimic increases breast cell migration and decreases CD9 protein levels. ( A ). Cell migration in 184A1 cells following transfection of a miR-518f-5p mimic assessed using transwell migration assay. ( B ). Migration of MDA-MB-231 cells following transfection of miR-518f-5p mimic. Migration assay results are shown as fluorescence intensity (arbitrary units), * p = 0.02, ** p = 0.002. ( C ). Proliferation of 184A1 cells using the resazurin assay, after transfection of miR-518f-5p mimic. ( D ). MDA-MB-231 proliferation following transfection of a miR-518f-5p mimic, as measured using resazurin assay. Cell proliferation results are shown as fold change fluorescence relative to 24 h fluorescence (arbitrary units). ( E ). Changes in cell adhesion to basement membrane extract (BME) and fibronectin (FN) were assessed using an adhesion assay, following transfection with miR-518f-5p mimic in 184A1 cells. ( F ). Cell adhesion was assessed in MDA-MB-231 cells following transfection of miR-518f-5p mimic. Adhesion results are expressed as a ratio of adhesion (fluorescence intensity of adherent cells compared to total seeded cells, arbitrary units), FN: * p = 0.04, BME: * p = 0.01, n = 3. ( G ). Total CD9 protein expression was measured using SDS-PAGE and western blotting, following transfection of a miR-518f-5p mimic in 184A1 (left) and MDA-MB-231 cells (right) for 72 h. ( H ). Representative western blots of CD9 total protein expression following transfection with miR-518f-5p mimic for 72 h. Results shown as CD9 total protein normalized to beta actin protein expression, n = 3, * p = 0.04, ** p = 0.001. See for full western blotting images.

Article Snippet: Cells were seeded in 96 well plates and transfected with the CD9 3’UTR or empty 3’UTR renilla reporter vector (SwitchGear Genomics, Menlo Park, CA, USA) and pmiR-Report firefly luciferase transfection control vector (Promega) using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA).

Techniques: Transfection, Migration, Transwell Migration Assay, Fluorescence, Resazurin Assay, Cell Adhesion Assay, Expressing, SDS Page, Western Blot